Fig. 2

The LCb478, but not LCb, fragments acquire paternal-allele-specific methylation in YAC–TgM. Tail somatic DNA from the lambda (A), LCb (B) or LCb478 (C), and DNA from 2-cell embryos of the LCb478 (C) YAC–TgM, inheriting the transgenes either paternally (pat.) or maternally (mat.), were analyzed by bisulfite sequencing. Methylated and unmethylated CpG motifs are shown as filled and open circles, respectively, and each horizontal row represents a single DNA template molecule. The results of 2-cell-embryo DNA from a single litter are presented together in a cluster. The numbers on the right of each row indicate number of times the pattern was observed by DNA sequencing