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Fig. 3 | Epigenetics & Chromatin

Fig. 3

From: SUMOylated non-canonical polycomb PRC1.6 complex as a prerequisite for recruitment of transcription factor RBPJ

Fig. 3

Changes in RBPJ binding depend on the presence of L3MBTL2 and E2F6. a ChIP-qPCR experiments showing binding of L3MBTL2 to regulatory elements of Notch target genes in HEK293 cells. CDC7-2Kb served as a negative control (CTRL). b ChIP-qPCR experiments showing binding of E2F6 to regulatory elements of Notch target genes in HEK293 cells. Gene Desert served as a negative control (CTRL). The mean of at least three technical replicates ± SD. c ChIP-qPCR analysis of L3MBTL2 binding at regulatory elements of Notch target genes in CRISPR/Cas9 mediated RBPJ depleted cells. d ChIP-qPCR analysis of E2F6 binding in CRISPR/Cas9 mediated RBPJ depleted cells in comparison with control cells. CDC7-2 Kb served as a negative control (CTRL). e ChIP-qPCR analysis of RBPJ binding at regulatory elements of Notch target genes in L3MBTL2 KO and f E2F6 KO cells in comparison with the control cells. Gene Desert served as a negative control (CTRL). The mean of at least three independent biological replicates ± SD

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Epigenetics & Chromatin

ISSN: 1756-8935