Fig. 2

Expression levels of menin proteins after transient transfections of HEK293 cells and or stable integration in stable HeLa cell lines. A Protein expression was assessed by ectopic expression of MEN1 WT and mutant cDNA in HEK293T cells and subsequent immunoblotting using menin antibodies or α-tubulin antibodies as a loading control. B Immunoblots upon doxycycline induction of stable HeLa cell lines showing expression of GFP-tagged menin using menin antibodies or α-tubulin antibodies as a loading control. C qPCR analysis of MEN1 RNA levels. C Fluorescence microscopy images of GFP-tagged WT menin and mutant proteins stained with anti-GFP antibody and counter-stained with DAPI