Fig. 3

Bisulfite pyrosequencing of F0 sperm. Bisulfite pyrosequencing of CpG sites initially identified via WGBS as significantly differentially methylated between LE sperm and controls for A Osm, B Gabrb2, C Pxylp1, D Ntng1, E Mtss1l. (top) CpG sites on the x-axis correspond to those that were identified as significant by WGBS, and the measured methylation level is on the y-axis. Each point represents one individual. (bottom) CpG sites are labeled on the x-axis, mean methylation levels are plotted on the y-axis ± SEM. CpG sites labeled with “*” on the x-axis correspond to those identified via WGBS that were validated in the “top” part of the panel and are labeled with the same CpG number. Black, controls; blue, EE; green,  LE. ^*p < 0.05, ^** p < 0.01, ^***p < 0.005