Fig. 1

Tissues and experimental design of the Hi-C study. Lenses were harvested from newborn (P0.5) mice and microdissected into epithelium and fiber cells (30 lenses x 2 replicates). Mouse ES cells were harvested near ~ 80% confluency (2.0 × 106 cells x 2 replicates). Cells were crosslinked and processed according to Arima’s library preparation protocol. Chromatin contact maps were generated using the ENCODE pipeline. Differential compartment A/B analysis was performed using dcHiC (see Materials and Methods). The principal component analysis (PCA) on compartmental eigenvectors shows that both lens cells are distinct from the ES cells