Fig. 1

CpA-TAP has poor affinity for CENP-C and altered post-translational modifications (PTM) signature. a) Native CpA protein and fusion CpA-TAP protein consisting of CpA + S protein + TEV cleavage site + Protein A. b) CoIF of HeLa cells transiently expressing CpA-TAP with native CENP-C. Scale bar = 5 μm. c) Immuno-precipitation of native CpA versus CpA-TAP (S-tag IP) (see Fig. S1a) followed by Western detection for CENP-C, and quantification of the ratio of CENP-C enrichment normalized against CpA ChIP. Error bar represent SEM. HC = heavy chain. d) Long TAU (L-TAU) Western comparing control HeLa cells and cells with transiently transfected CpA-TAP (merged panel below). e) AFM heights for IP’ed native CpA versus CpA-TAP nucleosomes. AFM measurements were done in air mode. Bulk = extracted input chromatin and α-S ChIP = immuno-precipitated CpA-TAP chromatin. Scale bar = 50 nm