Fig. 3
From: Native and tagged CENP-A histones are functionally inequivalent
ChIPseq analysis of native CpA-TAP in DAXX KO HeLa cells. a) Western confirmation that DAXX is knock-out and that CpA-TAP is esxpressed (rDAXX: recombinant DAXX protein, AbCam cat #ab131785). b) CoIF of CpA-TAP and native CENP-C during interphase and mitosis (left panel), and native CpA versus S-tag ChIP followed by CENP-C Western (right panel). Scale bar =ā5Ā Ī¼m. c) Venn diagram detailing total, centromeric, and non-centromeric hotspots for native CpA and CpA-TAP. d) Categorical dissection of native CpA versus CpA-TAP incorporated sites