Fig. 7
From: Native and tagged CENP-A histones are functionally inequivalent
Tagged and untagged histone variants differ in genome wide distribution and nucleosomal interactions. a) Previously reported H1.5 and H1.5-HA ChIPseq sites were compared to native CpA ChIPseq sites from this study. b) ChIP performed against native CpA, GFP-CpA, and HA-CpA mono-nucleosomes and probed for histone H1.5 (Invitrogen Cat #711,912). HC = heavy chain. c) Ratios of H1.5/native or tagged CpA, normalized against H1.5/native CpA, from 2–5 independent experiments. Error bars = SEM