Table 1 AFM measurements of various nucleosomal structures
From: Native and tagged CENP-A histones are functionally inequivalent
Nucleosome | n | Height (nm) | Diameter (nm) | Volume (nm3) |
|---|---|---|---|---|
Untransfected (ChIP) | 81 | 1.1 ± 0.1 | 13.2 ± 1.5 | 157 ± 46 |
Bulk (control) | 1667 | 2.5 ± 0.2 | 14.9 ± 1.3 | 446 ± 106 |
Bulk (CpA-TAP) | 984 | 2.3 ± 0.3 | 14.9 ± 2.2 | 420 ± 151 |
H3 (ChIP) | 113 | 2.4 ± 0.3 | 13.5 ± 1.4 | 352 ± 91 |
CpA (ChIP) | 1387 | 1.9 ± 0.2 | 13.9 ± 2.2 | 291 ± 112 |
CpA-TAP (ChIP) | 1180 | 1.8 ± 0.2 | 14.8 ± 2.2 | 318 ± 126 |