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Fig. 2 | Epigenetics & Chromatin

Fig. 2

From: FOSL1 is a key regulator of a super-enhancer driving TCOF1 expression in triple-negative breast cancer

Fig. 2

FOSL1 knockdown reduces TCOF1 level and TCOF1 SE activity. (A) RT-qPCR verified the knockdown of FOSL1 mRNA in HCC1806 cells and MCF10-DCIS cells. Data, mean ± SEM: ***, p < 0.001 (n = 3). (B) RT-qPCR verified the downregulation of TCOF1 mRNA in HCC1806 cells and MCF10-DCIS cells knocked down FOSL1. Data, mean ± SEM: *, p < 0.05; **, p < 0.01 (n = 3). (C) Western blot of FOSL1 and TCOF1 in FOSL1-depleted HCC1806 cells and MCF10-DCIS cells. (D) SE activity of TCOF1-E1 in HCC1806 and MCF10-DCIS expressing FOSL1 or Control shRNAs was measured by dual-luciferase reporter assay. Data, mean ± SEM: **, p < 0.01; ***, p < 0.001 (n = 3)

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Epigenetics & Chromatin

ISSN: 1756-8935