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Fig. 3 | Epigenetics & Chromatin

Fig. 3

From: FOSL1 is a key regulator of a super-enhancer driving TCOF1 expression in triple-negative breast cancer

Fig. 3

FOSL1 binds to TCOF1-E1 SE and upregulates TCOF1 expression level. (A) FOSL1 and isotype IgG ChIP-qPCR in FOSL1-depleted HCC1806 and MCF10-DCIS cells using primers amplifying TCOF1-E1 SE. Data, mean ± SEM: **, p < 0.01; ***, p < 0.001 (n = 3). (B) FOSL1 and isotype IgG ChIP-qPCR in HCC1806 and MCF10-DCIS cells with or without JQ1 treatment (1 µM). Data, mean ± SEM: ***, p < 0.001 (n = 3). (C) Western blot of FOSL1 and TCOF1 in FOSL1-depleted cells or JQ1-treated cells. (D) BRD4 and isotype IgG ChIP-qPCR in FOSL1-depleted HCC1806 cells and JQ1-treated HCC1806 cells. Data, mean ± SEM: *, p < 0.05; **, p < 0.01 (n = 3)

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Epigenetics & Chromatin

ISSN: 1756-8935