Fig. 1

Dynamics of H3K9 methylation and acetylation in rabbit embryos. (A) Immunofluorescence imaging of di-methylated (H3K9me2) and tri-methylated (H3K9me3) histone 3 lysine 9, along with acetylated histone 3 lysine 9 (H3K9ac), in embryos at embryonic days E3.0, E3.5, E4.0, E5.0, E6.0, and E6.6. Scale bars: 100 μm. (B) Immunofluorescence visualization of SOX2, H3K9me2, H3K9me3, and H3K9ac in E4.0 embryos, with DAPI counterstaining. Scale bars: 40 μm. (C) Violin plots representing quantification of fluorescent signals for H3K9me2, H3K9me3, and H3K9ac in SOX2⁺ cells. Each embryo is represented by a unique color. All cells were quantified separately, with the mean of each embryo indicated with a larger dot. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001; ns, not significant