Fig. 3

Epigenetic modulation in rabbit embryos post-blastocyst formation. (A) Schematic diagram of the experimental approach, detailing inhibitor treatment and subsequent analyses. (B-C) Immunolabeling of the H3K9me2 (B) and H3K9me3 (C) marks, as well as the H3K9ac mark (C), in cultured rabbit embryos, treated with and without the A366 inhibitor. DNA was counterstained with DAPI. The fluorescence intensity for each mark was quantified in all cells of the embryos. Scale bars: 100 μm. (D-E) Quantification of H3K9me2 and H3K9me3 marks in SOX2⁺ versus SOX2⁻ cell populations of A366-treated and control embryos. (F-G) Immunolabeling for the H3K9ac mark (F) in cultured rabbit embryos, treated with and without the UF010 inhibitor. DNA was counterstained with DAPI. Scale bars: 50 μm. The fluorescence intensity for each mark was quantified either across all embryos (F) or in SOX2⁺ versus SOX2⁻ cell populations of UF010-treated and control embryos (G). In the violin plots, each color represents a different embryo, with the mean of each embryo indicated as a larger dot. **, p < 0.01; ***, p < 0.001; ****, p < 0.0001; ns, not significant