Fig. 4

Impact of EHMT1/2 inhibition on cell lineage distribution in rabbit embryos. (A) Immunolabeling for the H3K9me3 mark along with SOX2, SOX17 or GATA3 in rabbit embryos treated with A366. (B-D) Quantification of total cell count (B), SOX2⁺, SOX17⁺, and GATA3⁺ cells per embryo (C), as well as their respective percentages within each embryo (D). Scale bars: 100 μm. Violin plots represent the distribution of all analyzed embryos, with each embryo identified by a larger dot. *, p < 0.05; ****, p < 0.0001; ns, not significant. Data were obtained from 4 to 5 different experiments for each combination of antibodies (H3K9me3 + SOX2, H3K9me3 + SOX17, and H3K9me3 + GATA3), including both treated and control embryos