Fig. 5

Impact of HDAC class I inhibition on cell lineage distribution in rabbit embryos. (A) Immunolabeling for the H3K9ac mark along with SOX2, SOX17, or GATA3 in rabbit embryos treated with UF010. (B-D) Quantification of total cell counts (B), and number of SOX2⁺, SOX17⁺ and GATA3⁺ cells per embryo (C), with the respective percentage of each cell type calculated for each embryo (D). Scale bars: 100 μm. Violin plots represent all analyzed embryos and their distribution, with each embryo identified by a larger dot. ****, p < 0.0001; ns, not significant). Four to five different experiments were performed for each combination of antibodies (H3K9ac + SOX2, H3K9ac + SOX17, and H3K9ac + GATA3), always including both conditions, with or without UF010