Fig. 6

Transcriptomic alterations following EHMT1/2 inhibition in rabbit embryos. (A) Heatmap representation of differentially expressed genes among EPI, PE and TE groups. (B) Two-dimensional UMAP representation of samples categorized by embryonic lineage. (C) Two-dimensional UMAP distinguishing samples based on both lineage and culture conditions. (D-F) Heatmap of differentially expressed genes between control and A366-treated samples over 48 h, specifically within the EPI (D), TE (E), and PE (F) lineages. In panels A, D, E and F, expression is shown in log2Ex scaled and log-normalized format. (G) The protein-protein interaction (PPI) network displays differentially expressed genes (DEGs) as seed nodes in blue and their top 10 ranking neighbors in grey for each cell lineage. Statistically significant correlations between DEGs are depicted as edges (grey lines). This analysis was conducted separately for downregulated and upregulated genes