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Fig. 3 | Epigenetics & Chromatin

Fig. 3

From: Acquired sperm hypomethylation by gestational arsenic exposure is re-established in both the paternal and maternal genomes of post-epigenetic reprogramming embryos

Fig. 3

Detailed annotation analysis of CpGs and DMCs of F2 embryos. A) Average methylation levels of all CpGs in the annotated regions. The differences in the methylation levels between the control and arsenic group were tested by Welch’s t test. B) Distribution (%) of detailed annotated regions among all CpGs (gray bars) and among all DMCs (hypoDMCs (black bars) plus hyperDMCs (white bars)). Methylation analysis was performed using methylKit with destrand = false. DMC was defined as q < 0.01 and methylation difference ≥ 10%. For each region, difference in the occurrence of CpGs in all CpGs, and hypoDMCs in all hypoDMCs or hyperDMCs in all hyperDMCs, was assessed by Fisher’s exact test. Significantly increased (up) or decreased (down) DMCs (p < 0.001) were marked with † for hypoDMCs and # for hyperDMCs. U and D represent an up or down-regulated occurrence compared to CpGs, respectively. C) Box-and-whisker plots of methylation levels of hypo/hyperDMCs in the annotated regions. Crosses and bars in the box represent average and median values, respectively. D) Number of hypo/hyperDMRs assigned in the individual annotated regions

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Epigenetics & Chromatin

ISSN: 1756-8935