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Table 1 The methylation levels of CpGs in the paternal and maternal genomes of F2 embryos *

From: Acquired sperm hypomethylation by gestational arsenic exposure is re-established in both the paternal and maternal genomes of post-epigenetic reprogramming embryos

Genomes

Number of CpGs

Methylation level of CpGs (%)

  

Control

Arsenic

p value**

F2 Embryo

Genome-unspecified

1,410,646

44.36

43.66

< 2.2 × 10− 16

Paternal

64,611

51.57

50.73

1.6 × 10− 8

Maternal

93,966

52.19

51.43

2.7 × 10− 9

F1 sperm

1,303,934

45.90

45.65

3.7 × 10− 6

  1. * An N-masked reference genome was produced based on the GRCm38/mm10 and N-masking at SNP positions between GRCm38/mm10 and C3H/HeN genomes. RRBS data of F2 embryos were mapped to the N-masked reference genome and paternal or maternal genome-specific splitting was performed by SNPsplit. For comparison, RRBS data of F1 sperm was also mapped to the N-masked reference genome. Please see the Methods section for the analysis conditions
  2. ** The difference between the methylation levels in the two groups was assessed by Welch’s t test
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Epigenetics & Chromatin

ISSN: 1756-8935