Fig. 3

Alteration of long LOCKs in normal and tumor cell lines. A Changes in the enrichment of H3K27me3 long LOCKs in common PMDs or HMDs between normal and tumor cell lines. B Tumor-loss, shared, and gain long LOCKs based on tumor and normal cell line pairs were identified. The stacked bar charts show the proportions of common PMDs, common HMDs, and non-common PMD/HMD regions covered by these three groups of LOCKs. C Distribution of tumor-loss, shared, and gain LOCKs in the NE2-KYSE450 pair across different genomic regions. D Average H3K27me3 intensity in the three groups of long LOCK regions and their surrounding regions for the NE2-KYSE450 pair. E Heatmap shows the average DNA methylation levels of the three groups of long LOCK regions in NE2 vs. KYSE450, across the whole genome and within common PMDs and HMDs. F Normalized gene expression levels within the three groups of LOCKs in common PMDs, for NE2 and KYSE450. G Consistent with F, but for oncogenes. The two-tailed t-test was used (***p < 0.001, **p < 0.01, *p < 0.05, NS = not significant) in (A, F and G)