Fig. 4

Changes in long LOCKs within S/I/L-PMDs in normal and tumor cells. A The enrichment of H3K27me3 long LOCKs in S/I/L-PMDs. The two-tailed t-test was used (***p < 0.001, **p < 0.01, *p < 0.05, NS. = not significant). B The proportions of the three groups of long LOCKs covering S/I/L-PMDs. C The heatmap shows average DNA methylation levels of the three groups of long LOCKs within S/I/L-PMDs in normal breast cell lines and Her2 + BRCA cell lines. D The heatmap shows H3K27me3 and H3K9me3 signals of three groups of long LOCKs within S/I/L-PMDs in normal breast cell lines and Her2 + BRCA cell lines. Each row represents a long LOCK region, with bars on the right indicating its PMD classification (S/I/L), the corresponding LOCK group, along with the change state of H3K27me3 and H3K9me3 in tumors compared to normal samples, with a minimum change of 0.1. The text on the far right shows the proportion of tumor-gain LOCKs in I- or L-PMDs with decreased, unchanged, or increased H3K9me3 signals. T, tumor; N, normal. E As an example, a snapshot of Integrated Genome Viewer (IGV) software is shown. It reflects the loss of H3K9me3 in a tumor-gain LOCK region within an L-PMD