Fig. 5

Short LOCKs are related to poised promoters. A Peak density within short or long LOCKs, indicating the percentage of the total peak length relative to the total LOCK length, in 109 Roadmap samples. B The heatmap shows the top 20 LOLA terms most significantly enriched in peaks within short LOCKs. Each column represents a peak type from a Roadmap sample, with color intensity showing the level of significance. C Comparing short LOCKs to the whole genome based on Roadmap samples. The top part shows the proportion of poised promoters among CGI promoters with H3K27me3 peak binding, while the bottom part shows the proportion of poised promoters overlapping with ESC poised promoters among all poised promoters. D The number of CGI promoters within tumor-loss, shared, and gain short LOCKs identified across different normal-tumor pairs. E A schematic illustrating the identification process of upregulated and hypermethylated CGI-promoter genes. F Number of CGI promoters within an upregulated or hypermethylated group. G In the NE2-KYSE450 pair, the H3K27me3 intensity, DNA methylation, gene expression, H3K27ac intensity, and H3K4me3 intensity of upregulated or hypermethylated CGI promoters. T, tumor; N, normal. The two-tailed t-test was used (***p < 0.001, **p < 0.01, *p < 0.05, NS. = not significant) in (A and G). H Percentage of poised promoters among upregulated or hypermethylated CGI promoters