Fig. 6

Identification of ETS1 as a key TF upregulated in tumor-loss short LOCKs. A Bubble plot shows motifs identified using Homer software with upregulated CGI-promoters as foreground and hypermethylated CGI-promoters as background. Motifs are ranked by their occurrence across multiple normal-tumor pairs. No significant motifs were found in the NE2 vs KYSE510 group. Bubble size reflects TF expression in the tumor cell line of each pair and color indicates FDR. B Counting motif subfamily occurrences in all normal-tumor pairs. C Normalized expression level of the ETS1 gene in different ESCC cell lines from the DepMap portal. TPM: transcripts per million. D IGV snapshots display the genomic environment of representative ETS1 target genes which are upregulated in the KYSE450 cell line compared to NE2. E, F Relative expression of ETS1 target genes detected by qRT-PCR after ETS1 knockdown in KYSE150 (E) and overexpression in T.T cells (F). Two-tailed t-test was used (***p < 0.001, **p < 0.01, *p < 0.05, NS. = not significant)