Fig. 3

The landscape of chromatin accessibility in normal and fibrotic mouse lung. (A) Genome-wide ATAC-seq consensus peaks in lung tissues. Each column represents one peak. The color represents the intensity of chromatin accessibility (red/more accessible and blue/less accessible). (B) Genomic distribution of consensus peaks across all samples. (C) Principal component analysis (PCA) of accessible loci as determined by ATAC-seq from control and fibrotic mice, either Sham operated and 21 days after intratracheal BLM delivery. Each dot represents an individual mouse. (D) Boxplots display principal component two across PBS samples (orange) and BLM samples (blue) for the study. (E) Volcano plot, visualizing the statistical difference of the differentially accessible regions. (F) Genomic distribution of differentially accessible regions between control and BLM-induced fibrotic mice. (G-H) Visualization of chromatin accessibility region of Mmp19 (G) and Bmp4 (H) in control and BLM-induced fibrotic mice. (I-J) Pathway enrichment analysis of differentially accessible regions between control and BLM-induced fibrotic mice. (K-L) De novo DNA motif analysis of differentially accessible chromatin sites between control and BLM-induced fibrotic mice