Fig. 2
From: LSD1 induces H3 K9 demethylation to promote adipogenesis in thyroid-associated ophthalmopathy
LSD1 knockdown can inhibit adipocyte differentiation. Expression of Inflammatory factors in normal orbital adipose cells and in orbital adipose cells derived from TAO patients (A),*p < 0.05, **p < 0.01, ***p < 0.001 according to the two-way ANOVA test, n = 3 biological repeats. Expression of adipose differentiation genes in normal human orbital adipose cells and in orbital adipose cell derived from TAO patients (B), *p < 0.05, **p < 0.01, ***p < 0.001 according to the two-way ANOVA test, n = 3 biological repeats. Western blot analysis of LSD1 protein level in non-TAO orbital adipose cells and TAO orbital adipose cells (C). Efficiency detection of lentivirus-mediated LSD1 knockdown in orbital adipose cells derived from TAO patients (D). Expression of adipose differentiation genes in TAO orbital adipose cells and TAO orbital adipose cells knocking down LSD1 (E), *p < 0.05, **p < 0.01, ***p < 0.001 according to the two-way ANOVA test, n = 3 biological repeats. Expression of inflammatory genes in TAO orbital adipose cells and TAO orbital adipose cells knocking down LSD1 (F),*p < 0.05, **p < 0.01, ***p < 0.001 according to the two-way ANOVA test, n = 3 biological repeats. Western blot analysis of UCP1, PGC1α proteins level in TAO orbital adipose cells and TAO orbital adipose cells knocking down LSD1 (G)