Fig. 4
From: LSD1 induces H3 K9 demethylation to promote adipogenesis in thyroid-associated ophthalmopathy
Role of LSD1-mediated adipogenesis in TAO cells. Western blot analysis of H3 K9 me2 protein level in non-TAO orbital adipose cells and TAO orbital adipose cells (A). Western blot analysis of H3 K9 me2 proteins level in TAO orbital adipose cells and TAO orbital adipose cells knocking down LSD1 (B). Western blot analysis of H3 K4 me2 proteins level in TAO orbital adipose cells and TAO orbital adipose cells knocking down LSD1 (C). The distribution of H3 K9 me2 modification in the promoter region of TAO orbital adipose cells and non-TAO orbital adipose cells (D). The distribution of H3 K9 me2 modification in the promoter region of TAO orbital adipose cells and TAO orbital adipose cells knocking down LSD1 (E). GO analysis genes with increased histone modification of H3 K9 me2 after LSD1 knockdown in TAO orbital adipocytes (F). Modification analysis of H3 K9 me2 on FABP4 gene locus in non-TAO orbital adipose cells, TAO orbital adipose cells and TAO orbital adipose cells knocking down LSD1 (G). Modification analysis of H3 K9 me2 on CCL18 gene locus in non-TAO orbital adipose cells, TAO orbital adipose cells and TAO orbital adipose cells knocking down LSD1 (H). Representative images of lipid droplet formation when treated with LSD1 inhibitor pargyline (from 1 to 6uM) in TAO cells (I) and the quantification of lipid droplets (J). scale bar: 50 μm. *p < 0.05, **p < 0.01, ***p < 0.001 according to the one-way ANOVA test, n = 4 biological repeats