Skip to main content
Fig. 2 | Epigenetics & Chromatin

Fig. 2

From: MSH2 is not required for either maintenance of DNA methylation or repeat contraction at the FMR1 locus in fragile X syndrome or the FXN locus in Friedreich’s ataxia

Fig. 2

(A) A schematic showing the creation of MSH2 null fragile X syndrome human embryonic stem cell lines (FXS hESCs). FXS ESCs (WCMC37F) were transfected with plasmids expressing nCas9 and dual guide RNAs targeting MSH2 exon 3. Edited clones were screened using western blot and DNA sequencing, and MSH2 null clones were selected for further study. (B) Western blot showing MSH2 expression in two cell lines each for FXS MSH2 WT and FXS MSH2 KO. β-actin is used as a loading control. Western blot of all clones assayed is shown in Figure S1B. (C) Immunofluorescence imaging for MSH2 (green) shows a strong, nuclear signal in FXS MSH2 WT-2 and the absence of MSH2 in FXS MSH2 KO-2 cells. Scale bars represent 300 μm

Back to article page

Epigenetics & Chromatin

ISSN: 1756-8935